Requests using FY24 funds are no longer being accepted. Requests for using FY25 funds can be submitted and the funds will be transferred in FY25.
SomaScan Assay profiles more than 7,000 proteins in only 55 µL of sample. The assay runs on Tecan’s Fluent liquid handling platform and utilizes a unique set of capture reagents called Slow Off rate Modified Aptamers (SOMAmers) to measure the relative abundance of proteins in a multitude of biological matrices including human serum, plasma, cerebrospinal fluid, urine as well as many others. Through a unique reagent binning design, the assay is capable of spanning 10 logs of dynamic range. The platform is high throughput and is capable of running 81subject samples at a time. In addition, it is highly reproducible, and the median %CV in replicate samples is typically <5%. It has proved widely successful when screening for biomarker signatures in a variety of biomedical applications, such as those predicting patient outcomes or characterizing responses to interventions and therapies. This is typically achieved when utilizing longitudinal samples from individuals, and with study designs including discovery and validation cohorts.
Who Can Use This Resource
This technology is available to NIH researchers through the Center for Human Immunology, Inflammation, and Autoimmunity (CHI) Technical Partnership Program. This program requires investigators to purchase the required SomaScan reagents that will be processed by NIAID SomaScan technicians.
How To Access
Requests for utilizing the SomaScan platform can be submitted in the RTB.NIH.GOV system.
For inquiries contact Brian Sellers.
Location
The SomaScan V4.1 Assay is performed on the Tecan Fluent instrument that is located at the Center for Human Immunology, Inflammation, and Autoimmunity located in Building 10 on the Bethesda campus.
Research Network Affiliation
NIH Center for Human Immunology, Inflammation, and Autoimmunity
The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is an affiliate of the National Institute of Allergy and Infectious Diseases Research Technologies Branch. CHI staff collaborate with researchers to apply advanced technologies to achieve an integrated and predictive understanding of human immunity, immune-microbiome behavior and their function in health and disease.
For inquiries about the Center for Human Immunology, Inflammation, and Autoimmunity contact:
Rachel Tracy
Executive Assistant