Genomics—Integrated Research Facility at Fort Detrick

The Genomics team at the IRF-Frederick supports studies, including virus genome sequence analysis, host transcriptome and metagenome analysis, and subpopulation or immune response analysis using single-cell sequencing. The team provides collaborative support for genomic analysis planning, sample preparation, preparation of sequencing libraries, next-generation sequencing using short-read or nanopore-based long-read sequencing, and data analyses.

Main Areas of Focus

• Virus genome sequence assembly and variant analysis
• Host transcriptome analysis
• Single-cell sequencing

Capabilities

• Next-generation sequencing (including sample processing and library preparation)
  • Total RNA sequencing (bulk)
  • Single-cell RNA sequencing
  • Whole-genome sequencing
  • Amplicon sequencing
  • Nanopore-based long-read sequencing
  • Immune repertoire profiling – V(D)J sequencing for T cell receptor and B cell receptor (TCR/BCR) analysis
• Data analysis:
  • Variant analysis
  • Differential gene expression
  • Single-cell RNAseq analysis
  • Metagenomic analysis

Specialized Equipment

• Illumina NextSeq2000
• Illumina MiSeq
• Illumina iSeq100
• Oxford Nanopore Technologies MinION
• PerkinElmer SciClone G3 NGSx
• 10X Genomics Chromium Controller
• 10X Genomics Chromium iX
• Agilent 4200 TapeStation
• Qubit Flex Fluorometer
• Miltenyi Biotec gentleMACS Octo Dissociator
• Nexcelom Cellometer Auto 2000

Lirong prepares libraries for sequencing using PerkinElmer SciClone G3 NGSx in the BSL-2 laboratory

Credit: NIAID IRF-Frederick

Shawn sets up a sequencing run on the Illumina NextSeq2000 in the BSL-2 laboratory

Credit: NIAID IRF-Frederick

Location

Integrated Research Facility at Fort Detrick (IRF-Frederick)