Located at Rocky Mountain Laboratories (RML) in Hamilton, Montana and the NIH main campus in Bethesda, Maryland, the Research Technologies Branch (RTB) Genomics Research Section (GRS) enables intramural NIAID investigators to use state-of-the-art applications in gene expression and sequencing technologies in their research programs. Our staff provides expertise, instrumentation, and data analysis and interpretation.
Computer generated model depicting RNA polymerase
Major Areas of Support
- Next-generation DNA/RNA sequencing
- Single cell RNA processing and analysis
- Spatial genomics
- Long read DNA sequencing
- TaqMan (RT-qPCR) analysis
- Droplet Digital PCR
Instrumentation
Rocky Mountain Laboratories
- Illumina MiSeq
- Illumina NextSeq 2000
- Oxford Nanopore MinION
- 10x Genomics Chromium X
- 10x Genomics CytAssist
- Applied Biosystems QuantStudio
- Bio-Rad Droplet Digital PCR
Bethesda
- Illumina NextSeq 2000
- 10X Genomics Chromium single-cell sequencing
Selected Publications
Intranasal immunization with avian paramyxovirus type 3 expressing SARS-CoV-2 spike protein protects hamsters against SARS-CoV-2.
Park HS, Matsuoka Y, Luongo C, Yang L, Santos C, Liu X, Ahlers LRH, Moore IN, Afroz S, Johnson RF, Lafont BAP, Dorward DW, Fischer ER, Martens C, Samal SK, Munir S, Buchholz UJ, Le Nouën C. NPJ Vaccines. 2022 Jun 28;7(1):72. doi: 10.1038/s41541-022-00493-x.PMID: 35764659
A single intranasal dose of a live-attenuated parainfluenza virus-vectored SARS-CoV-2 vaccine is protective in hamsters.
Liu X, Luongo C, Matsuoka Y, Park HS, Santos C, Yang L, Moore IN, Afroz S, Johnson RF, Lafont BAP, Martens C, Best SM, Munster VJ, Hollý J, Yewdell JW, Le Nouën C, Munir S, Buchholz UJ. Proc Natl Acad Sci U S A. 2021 Dec 14;118(50):e2109744118. doi: 10.1073/pnas.2109744118.PMID: 34876520
Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer.
Avanzato VA, Matson MJ, Seifert SN, Pryce R, Williamson BN, Anzick SL, Barbian K, Judson SD, Fischer ER, Martens C, Bowden TA, de Wit E, Riedo FX, Munster VJ. Cell. 2020 Dec 23;183(7):1901-1912.e9. doi: 10.1016/j.cell.2020.10.049. Epub 2020 Nov 4.PMID: 33248470
Therapeutic responses to Roseomonas mucosa in atopic dermatitis may involve lipid-mediated TNF-related epithelial repair.
Myles IA, Castillo CR, Barbian KD, Kanakabandi K, Virtaneva K, Fitzmeyer E, Paneru M, Otaizo-Carrasquero F, Myers TG, Markowitz TE, Moore IN, Liu X, Ferrer M, Sakamachi Y, Garantziotis S, Swamydas M, Lionakis MS, Anderson ED, Earland NJ, Ganesan S, Sun AA, Bergerson JRE, Silverman RA, Petersen M, Martens CA, Datta SK. Sci Transl Med. 2020 Sep 9;12(560):eaaz8631. doi: 10.1126/scitranslmed.aaz8631.PMID: 32908007
Chromoanasynthesis as a cause of Jacobsen syndrome.
Anzick S, Thurm A, Burkett S, Velez D, Cho E, Chlebowski C, Virtaneva K, Bruno D, Martin CB, Lang DM, Brooks B, Martens C, McDermott DH, Murphy PM. Am J Med Genet A. 2020 Nov;182(11):2533-2539. doi: 10.1002/ajmg.a.61824. Epub 2020 Aug 25.PMID: 32841469
SARS-CoV-2 infection dynamics in lungs of African green monkeys.
Speranza E, Williamson BN, Feldmann F, Sturdevant GL, Pérez-Pérez L, Mead-White K, Smith BJ, Lovaglio J, Martens C, Munster VJ, Okumura A, Shaia C, Feldmann H, Best SM, de Wit E. bioRxiv. 2020 Aug 20:2020.08.20.258087. doi: 10.1101/2020.08.20.258087. Preprint.PMID: 32839775
Access
Currently, collaborating with RTB is only available to federally funded institutions. Researchers may access all RTB support services by visiting RTB on Inside NIAID (this link is only available to NIAID lab scientists).
Leadership
Craig Martens, Ph.D
Chief, Genomics Research Section
Craig Martens, Ph.D.